LC3A/LC3B Polyclonal Antibody
简要描述：LC3A/LC3B Polyclonal Antibody同义词MAP1LC3B;ATG8F;LC3B;MAP1A/1BLC3;MAP1LC3B-a;MAP1LC3A;ATG8E;LC3;LC3A;MAP1ALC3;MAP1BLC3;LC3A/LC3B反应物种Human, Mouse, Rat宿主物种Rabbit
产品型号： PAB34124
所属分类：多克隆抗体
更新时间：2023-05-04
详细说明：
LC3A/LC3B Polyclonal Antibody
产品信息
同义词
MAP1LC3B;ATG8F;LC3B;MAP1A/1BLC3;MAP1LC3B-a;MAP1LC3A;ATG8E;LC3;LC3A;MAP1ALC3;MAP1BLC3;LC3A/LC3B
免疫原
A synthetic peptide of human LC3A(NP_115903.1).
推荐稀释比
WB,1:500 - 1:2000|IHC,1:50 - 1:200|IF/ICC,1:50 - 1:200
illustration
Western blot analysis of extracts of various cell lines, using LC3A/LC3B antibody at 1:500 dilution.293T cells were treated by Chloroquine (50 μM) at 37℃ for 20 hours.NIH/3T3 cells were treated by Chloroquine (50 μM) at 37℃ for 20 hours.C6 cells were treated by Chloroquine (50 μM) at 37℃ for 20 hours.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Enhanced Kit .
Exposure time: 3min.|Immunohistochemistry of paraffin-embedded human colon carcinoma using LC3A/LC3B Rabbit pAb at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.|Immunohistochemistry of paraffin-embedded mouse testis using LC3A/LC3B Rabbit pAb at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.|Immunohistochemistry of paraffin-embedded rat brain using LC3A/LC3B Rabbit pAb at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.|Immunofluorescence analysis of 293T Treated with Chloroquine and 293T cells using LC3A/LC3B Rabbit pAb at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
该品牌专注于抗体平台建设12年，引进抗体制备技术，不断优化抗体平台，拥有小鼠单克隆抗体产品线、兔多克隆抗体产品线以及兔单克隆抗体产品线，投放市场的抗体达到10000多种，广泛的应用于IHC、IF、Western Blot、ELISA、ICC/IF、Flow Cyt等科研领域，这些产品已经覆盖了人类基因组靶标；一抗过5000种抗体经过KD/KO验证。
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备注：图片仅供参考。仅供科研，不可用于临床。
